Caller Sure. It’s 610‐492‐1243. Operator That’s 610‐492‐1243? Caller Yes. Operator And Mr. Simpson, are you over the age of 25?, the overall cost to own a new car in 2022. The overall average cost to own and operate a new car in 2022 is $10,728. Category Costs The costs for each of these categories as listed below are an average calculated over five years and 75,000 miles. Average Ownership Costs Per Mile Miles per Year 10k 15k 20k Average Cost 76¢ 72¢ 70¢, The clubs that we studied span various car brands and, in a majority of cases, are individual car clubs that are orga-nized around a particular corporate brand (e.g., Ford, Volk-swagen) rather than a specific product family or make. These clubs range from about a dozen members to hundreds of members. Moreover, as do HOGs, most car clubs receive, αCD19˙CAR Day 3 Day 7 Day 9 Day 16 0.31% 13.27% 27.69% 3.71% Leukapheresis Lymphodepletion CAR T cells s Lymphodepletion CAR T cells Rituximab Tacrolimus Cyclophos-phamide Belimumab , activation in CD28-YMFM CAR-T cells relative to CD28 CAR-T cells and decreases in VAV1, PLCγ1, and ERK activation (Figure 3, A–E). AKT activation was significantly increased in CD28-YMFM CAR-T cells generated from 4 different healthy donors compared with CD28 CAR-T cells produced from the same donors (Figure 3, D and E)., .